In Gavi-eligible countries partnerships are dynamic networks of immunization actors who work together to support all stages and aspects of Gavi support. This paper describes a conceptual framework--the partnership framework--and analytic approach for evaluating the perceptions of partnerships’ value as well as the results from an application to one case in Uganda. We used a mixed-methods case study design embedded in the Gavi Full Country Evaluations to test the partnership framework on Uganda’s human papillomavirus vaccine application partnership. The partnership was not perceived to have increased the efficiency of the process, perhaps as a result of unclear or absent guidelines around roles and responsibilities. We concluded that the health and functioning of global health partnerships can be evaluated using the framework and approach presented here. Network theory and methods added value to the conceptual and analytic processes, and we recommend applying this approach to other global health partnerships to ensure that they are meeting the complex challenges they were designed to address.